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a would be the acid dissociation consistent. pH-fee profiles that confirmed the loss of catalytic exercise at large and lower values of pH ended up in shape to equation 4, the place K
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3 experimental probes had been marshaled with NagA to unveil link naga26 the supply of the rate limitation for this enzyme. The primary of these experiments as opposed the costs of substrate hydrolysis in H2O and D2O. Applying Zn-NagA with N
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